The video was taken from the ATL Lounge, where police found three people suffering from gunshot wounds in the early hours of July 23.
Police believe an argument in the living room led to the shooting.
Two of the victims, 25-year-old Toronto resident Tosin Amost-Arowoshegbe and 22-year-old Mississauga resident Chibueze Momah, were pronounced dead at the scene.  Police said the two men worked as security guards at the facility located near Highway 7 and Keele Street.
A third victim, a 20-year-old patron, was taken to hospital, where she is in stable condition.
Police said the shooting was a targeted incident.
Video released Wednesday shows the suspect at the club as well as someone believed to be known to him.
Police are asking for help identifying the suspect in the video.  He is described as a black man in his 20s with a thin build and was wearing a white and black T-shirt.
Police continue to ask anyone with cellphone video or photos from inside and outside the club or other footage from the area to upload it to a special link.  Anyone with information about the incident can also contact the homicide unit at 1-866-876-5423 ext.  7865 or Crime Stoppers anonymously at 1-800-222-TIPS.